ʻO ka Media Culture He Paʻi no ka hoʻomohala ʻana
ʻO ka media moʻomeheu cell he hupa meaʻai i loaʻa nā meaʻai pono a me nā mea ulu e pono ai no ka ulu a mālama ʻana.ʻO ka mea maʻamau ka mea i hoʻohui ʻia me ka hui pū ʻana o nā carbohydrates, proteins, lipids, minerals, vitamina, a me nā mea ulu.Hāʻawi pū ka media i kahi kaiapuni maikaʻi no nā cell e ulu ai, e like me ka pH maikaʻi loa, ka osmotic pressure, a me ka mahana.Loaʻa paha i ka media nā lāʻau antibiotic no ka pale ʻana i ka maʻi bacteria a i ʻole fungal contamination, a me nā mea hoʻohui ʻē aʻe e hoʻonui ai i ka ulu ʻana o nā ʻano kelepona kikoʻī.Hoʻohana ʻia ka media moʻomeheu cell i nā noiʻi like ʻole a me nā noi lapaʻau, e like me ka ʻenekinia kiko, ʻike lāʻau lapaʻau, a me ka noiʻi maʻi maʻi.
Kūʻai Kūʻai Kūʻai Kūʻai
ʻO ka media moʻomeheu stem cell ka hui pū ʻana o kahi ʻano basal, e like me Dulbecco's Modified Eagle Medium (DMEM) a i ʻole RPMI-1640, a me kahi hoʻohui serum, e like me ka fetal bovine serum (FBS).Hāʻawi ka basal medium i nā meaʻai pono a me nā huaora, aʻo ka serum supplement i hoʻohui i nā mea ulu, e like me ka insulin, transferrin a me selenium.Eia kekahi, loaʻa paha i ka media moʻomeheu stem cell nā lāʻau antibiotic, e like me ka penicillin, e pale ai i ka hoʻopōʻino ʻia e ka bacteria.I kekahi mau hihia, hiki ke hoʻohui ʻia nā mea hoʻohui hou, e like me nā kumu ulu hou, i ka media moʻomeheu e hoʻonui ai i ka ulu ʻana o nā cell stem a i ʻole ka ʻokoʻa.
Pūnaehana Puʻupuʻu Embryonic Kanaka
ʻO nā pūnaewele pūpū embryonic (ESCs) nā pūnaewele kumu i loaʻa mai i ka nui o ke keena o loko o kahi blastocyst, kahi embryo preimplantation mua.Ua kapa ʻia nā ESC kanaka he hESCs.He pluripotent lākou, ʻo ia hoʻi, hiki iā lākou ke hoʻokaʻawale i nā ʻano cell āpau o nā papa germ mua ʻekolu: ectoderm, endoderm a me mesoderm.He mea waiwai nui lākou no ke aʻo ʻana i ka biology developmental, a ʻo kā lākou hiki ke hoʻohana i ka lāʻau lapaʻau hou e mālama ai i nā ʻano maʻi ākea ka mea nui o ka noiʻi.